|
|
|
|
|
| Rocha,Bruno Ribeiro; Narduche,Lorena; Oliveira,Clara Slade; Martins,Gabriel; Lilenbaum,Walter. |
| ABSTRACT: For a long time, it has been stated that urine leptospiral shedding is intermittent, which was observed primarily by culturing. However, culturing presents serious limitations, mainly low sensitivity, and failure on detection of leptospires cannot be neglected. PCR presents several advantages, mainly higher sensitivity. The present study aimed to analyze the occurrence of intermittency on leptospiral shedding by PCR in naturally and experimentally infected animals. In this study two experiments were conducted, the first with 60 cows naturally infected from an endemic herd. The second one was conducted in three sheep experimentally infected, each one with a different strain of Leptospira (strains Copenhageni L1-130, Canicola LO-4 and Pomona... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cows; Intermittent; PCR; Sheep; Strain. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000800453 |
| |
|
|
| Vasconcelos,Carlos Otávio de Paula; Brandão,Felipe Zandonadi; Martins,Gabriel; Penna,Bruno; Souza-Fabjan,Joanna Maria Gonçalves de; Lilenbaum,Walter. |
| ABSTRACT: This study evaluated the presence of vaginitis and the bacterial load associated with different intravaginal implants in ewes. Twenty-four Dorper and crossbred ewes were allocated into three groups and received intravaginal implant containing 0.3g progesterone (CIDR(r)), 60mg MAP or sponges without progesterone (CONTROL) for six days. Then, CIDR and MAP treated-ewes received 12.5mg dinoprost and 300IU eCG. Vaginal mucus samples were collected at four times: before device insertion, at the day of its removal, 24 and 48 hours after. The samples were cultured and the colonies were counted (CFU/mL) and identified. The results obtained from the counting of CFU mL-1 were submitted to Kruskal-Wallis H test, with P<0.05 being considered significant.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Intravaginal device; Microorganism; Sheep; Vaginal infection.. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000400632 |
| |
|
| |
|
|
|
